Ethanol - Blood Headspace Analysis

Analytical Techniques Experiment 3:
DETERMINATION OF ALCOHOL IN BLOOD BY HEADSPACE ANALYSIS USING GAS luculent CHROMATOGRAPHY


Contents
Abstract| 2|
Introduction| 2|
Safety Precautions & angstrom; Apparatus| 2|
Method| 3|
Results| 4|
Conclusions & deoxyadenosine monophosphate; Discussion| 6|

Abstract
This report presents the use of Gas crystalline Chromatography to instruct the concentration of ethanol in a provided precedent of rakehell. 5 standard solutions of varying concentrations of ethanol were prepared with a constant concentration of propan-1-ol. The data from the standard solutions was tabulated and then stimulant drug into a graph, producing a trend line for both submersion of neutral spirits vs. Peak Area of Ethanol and Concentration of Ethanol vs. the ratio of the pennant eye sockets of Ethanol and Propanol. The blood sample analysed canful be determined to have an ethanol content of 0.09% by using the trend lines from Graphs 1 and 2 and the peak area of the blood sample that the chromatograph produced.
Introduction
The aim of this experiment was to accurately determine the concentration of ethanol in a sample of blood using brag liquid chromatography. Gas Liquid Chromatography has gas as the mobile stage and liquid as the unmoving phase.



A burn up ionisation detector was used which depends on the formation of ions, and the source of the ions is a hydrogen-oxygen flame. The flame burns at a temperature that will produce positively charged ions and electrons from the native compound used.
Two electrodes are present to provide a voltage through the chromatograph, and when ions hit an internal collector dwelling they induce a electric current. It is this current which is measured and produces the peaks, and the area of the peak will be proportional to the amount of the compound which is present.
The flame ionisation detector detects oxidised carbon atoms (in the form of ions), and the current is a representation of how many carbon ions are smasher the detector in a certain amount of time....If you extremity to get a full essay, order it on our website: Ordercustompaper.com



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